Lunar Knights takes place on a version of Earth that is completely different from our own. Thanks to a powerful weapon called the paraSOL, vampires have managed to conquer the planet and engulf it in darkness. This has caused the world to become a hard, desolate place where people would rather become slaves than scratch out an existence. However, there are people out there who are fighting to change that and end the vampires' dark reign.
Like Boktai, Lunar Knights offers a combination of gameplay styles. Throughout the game you play as two characters, each offering a completely different style than the other. Lucian is a vampire bounty hunter who wields a powerful sword named Vanargand who has made it his duty to hunt down and destroy all of the top vampires. The other character is Aaron, a rookie gunslinger, a sort of vampire hunter who wields Knight, a gun that is able to draw on the sun’s power for ammo. Both are accompanied by elementals who offer hints throughout the game, as well as giving them special powers when merged with the two boys.
Defeating one of the master vampires isn’t enough, you must also purify them using sunlight. However, because of the paraSOL, there isn’t enough light reaching the Earth. Instead you must take them into orbit where they can be hit with the solar cannon, which is sort of like the Death Star of solar-powered guns. This opens up a space shooter mini-game that is interesting, but doesn’t quite match the quality of the rest of the game.
These shooter sequences make complete use of the DS's touch screen. Dragging the stylus across the screen moves your ship, while tapping on enemies fires its cannons. The problem with the set up is that you can’t fire and move at the same time, which brings up some issues later on when you’re attacked by multiple ships.
Between levels, you can visit an old scientist who will upgrade your weapons. The scientist can also alter an area’s climate based on the spirits you give him. Changing the climate of an area can unlock new paths in levels, leading to new treasures and challenges.